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When a fairy broke a magic mirror, causing the pieces of the mirror to be scattered into everyone's hearts, Kai saw things that he didn't need or want to see. It is assumed that the story follows the same or similar events from the original tale: The Snow Queen. If the day ends without rescuing the frozen employee, they will die.Īn employee wearing the EGO Suit from The Firebird will instantly be killed upon entering The Snow Queen's containment unit. If The Snow Queen wins: Both the rescuer and frozen employee are killed by her.Can be replaced by other Cheek slot E.G.O. Both employees will get a frozen mark near their face, neck and body, which will increase their Fortitude/ Max HP and Prudence/ Max SP by 6 points (Acts as an E.G.O. 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Inoculate labeled empty Petri dish with specified mL (0.1 or 1.0 mL) of diluted specimen.Prepare the dilution of the test sample expected to contain between 30-300 CFU/mL.01, 1.0 and 2.0 mL) Procedure of Pour plate technique Plate count agar (PCA) or nutrient agar.It has the advantage of not requiring previously prepared plates and is often used to assay bacterial contamination of foodstuffs. ![]() The pour plate technique can determine the number of microbes/mL in a specimen. ![]() On average, it will give a lower count as heat-sensitive microorganisms may die when they come in contact with a hot molten agar medium. The pour plate method of counting bacteria is more precise than the streak plate method. To ensure a countable plate, plate a series of dilutions. The number of microorganisms present in the particular test sample is determined using the formula:ĬFU/mL= CFU * dilution factor * 1/aliquotįor accurate counts, the optimum count should be within 30-300 colonies/plate. 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